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KMID : 0388420000100010083
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Konkuk Journal of Medical Sciences
2000 Volume.10 No. 1 p.83 ~ p.90
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Retroviral-mediated Gene Transduction of Human Gene "CD59" into Hepatocytes of Mice
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Kim Joon-Buhm
Choi Jai-Koo
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Abstract
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One of the major problems in the transplantation of vascularized organs such as kidney, liver, heart, lung, and pancreas is the difficulty in providing sufficief4it human donor organs to the large number of patients awaiting a transplant. Accordingly, mechanical devices or organs from other species, ie. xenotransplantation have been tested as an alternative source. However, there is a critical immunological barrier in xenotransplantation, too; rapid rejection processes mediated by natural antibodies and complement occur. Recently, some membrane proteins have been shown to be able to protect human cells from autologous complement activation and cell lysis. One of those is CD59 that, as a complement regulatory protein, blocks or inhibits the membrane attack complex. It has been hypothesized that the expression of CD59 on the transplanted organ cells may prevent or reduce the occurrence of hyperactive rejection. Therefore, as a step to test this hypothesis, we first investigated in this . study whether human CD59 can be successfully expressed in mouse primary hepatocytes by using a recombinant retroviral vector system.
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KEYWORD
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xenotransplantation, CD59, transduction
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